Journal: Pharmaceutics

Article Title: Biological Features of Nanoparticles: Protein Corona Formation and Interaction with the Immune System

doi: 10.3390/pharmaceutics14122605

Figure Lengend Snippet: Dysopsonins.

Article Snippet: Possible methods include modifying the surface of NPs with different types of coatings (polymers, proteins, or biomimetic coatings) and reducing the number of proteins bound to the surface, e.g., by creating a protein shield or increasing the binding of dysopsonins (using proteins that promote bypassing phagocytosis) [ ].

Techniques: Activation Assay, Liposomes, Inhibition

Journal: Pharmaceutics

Article Title: Biological Features of Nanoparticles: Protein Corona Formation and Interaction with the Immune System

doi: 10.3390/pharmaceutics14122605

Figure Lengend Snippet: Methods to avoid immune system engulfment of NPs. ( a ) PEG; ( b ) POx; ( c ) zwitterionic polymers; ( d ) pre-coating of NPs with dysopsonins; and ( e ) coverage of NPs with membranes derived from cells. POx: poly(2-Oxazoline); RBC: red blood cell. The figure was partly generated using Servier Medical Art by Servier, licensed under the Creative Commons Attribution 3.0 Unported Licence.

Article Snippet: Possible methods include modifying the surface of NPs with different types of coatings (polymers, proteins, or biomimetic coatings) and reducing the number of proteins bound to the surface, e.g., by creating a protein shield or increasing the binding of dysopsonins (using proteins that promote bypassing phagocytosis) [ ].

Techniques: Derivative Assay, Generated

Journal: Cells

Article Title: Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells

doi: 10.3390/cells10020466

Figure Lengend Snippet: Identifying attachment substrates necessary for human adipose stem cells’ (hASCs) adherence to cell culture surface, spreading, and growth under serum-free conditions. Human ASCs obtained from three different donors were used to screen a 96-well plate coated with 24 different peptides non-covalently linked to four different highly sulphated glycosaminoglycans (GAGs: synthetic dextran sulfate, heparin, chondroitin, and dermatan) [ , ]. The cells’ growth in the 96-well plate was evaluated using a commercial colorimetric cell viability assay. ( A ) Values of relative absorbance measured at 450 nm and classified according to the four types of sulphated GAGs (synthetic dextran sulfate, heparin, chondroitin, and dermatan) were used as a backbone for the formation of the different coating matrices. The four GAGs do not have a great influence on cell attachment and growth. ( B ) Ten different peptides non-covalently linked to highly sulphated GAGs strongly influence the ability of hASCs to attach and grow in the test micro-wells. The results obtained with the 14 poor-performing peptide-combinations are not shown. As in A, cell proliferation was measured using a commercial colorimetric kit. Histograms show the means ± standard deviations of the measured relative absorbances for hASCs obtained from three different donors. FN = fibronectin; VN = vitronectin; BS = bone sialoprotein; OP = osteopontin peptide; BMP2 = bone morphogenetic protein; PP = perlacan peptide; ColI = collagen I peptide; FGF = fibroblast growth factor.

Article Snippet: For this reason, we routinely culture hASCs on Corning PureCoatTM ECM Mimetic Fibronectin Peptide plasticware [ ], and all experiments of this study were performed using cells grown and expanded in serum-free conditions on this type of vessels.

Techniques: Cell Culture, Viability Assay, Cell Attachment Assay